Biological Buffers

Thermo Scientific™ DNA Gel Loading Dye (6X)

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving.

Invitrogen™ Potassium Acetate (3 M), pH 5.5, RNase-free

Invitrogen™ Potassium Acetate (3 M), pH 5.5, RNase-free

Invitrogen Molecular biology grade, 3M Potassium Acetate, pH 5.5 solution is supplied in one bottle containing 100 mL.

Cytiva Pharmalyte™ Carrier Ampholytes

Cytiva Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. Cytiva Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free

Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free

Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.

Thermo Scientific Chemicals Laemmli SDS sample buffer, reducing (6X)

Thermo Scientific Chemicals Laemmli SDS sample buffer, reducing (6X)

Laemmli SDS sample buffer is used to prepare protein samples in the Laemmli SDS-PAGE system.

Invitrogen™ Tris (1 M), pH 8.0, RNase-free

Invitrogen™ Tris (1 M), pH 8.0, RNase-free

Invitrogen Molecular biology grade, 1M Tris, pH 8.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), <i>Contains 1 ea. LA0041, NP0004, NP0005, NP0007</i>

Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007

Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.

Invitrogen™ 4X Bolt™ LDS Sample Buffer

Invitrogen™ 4X Bolt™ LDS Sample Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats

Gibco™ HEPES (1M)

Gibco™ HEPES (1M)

A zwitterionic organic chemical buffering agent commonly used in cell culture media

Invitrogen™ 20X Bolt™ MES SDS Running Buffer

Invitrogen™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats

Invitrogen™ BSA, acetylated (20 mg/mL)

Invitrogen™ BSA, acetylated (20 mg/mL)

Invitrogen Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is essential.

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

Invitrogen™ Tris-Glycine Transfer Buffer (25X)

Invitrogen™ Tris-Glycine Transfer Buffer (25X)

Made with high-purity reagents and are strictly quality controlled

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

Thermo Scientific™ MgCl<sub>2</sub> (magnesium chloride) (25 mM)

Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM)

Optimize your PCR reaction conditions with this Magnesium Chloride (MgCl2) solution.

Invitrogen™ Sodium Acetate (3 M), pH 5.5, RNase-free

Invitrogen™ Sodium Acetate (3 M), pH 5.5, RNase-free

Invitrogen Molecular biology grade, 3M Sodium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.

Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade

Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

Thermo Scientific Chemicals HEPES hemisodium salt

Thermo Scientific Chemicals HEPES hemisodium salt

CAS: 103404-87-1 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

Invitrogen™ 10X Bolt™ Sample Reducing Agent

Invitrogen™ 10X Bolt™ Sample Reducing Agent

Optimized for use with Bolt™ Bis-Tris Plus gels, and available in a variety of formats.

Lonza™ Ficoll™ DNA Loading Buffer, 6X

Lonza™ Ficoll™ DNA Loading Buffer, 6X

Ideal for improving resolution and separation of DNA fragments

Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)

Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

Thermo Scientific Chemicals MES, 99+%, <1% water

Thermo Scientific Chemicals MES, 99+%, <1% water

CAS: 4432-31-9 Molecular Formula: C6H13NO4S Molecular Weight (g/mol): 195.233 MDL Number: MFCD00006181 InChI Key: SXGZJKUKBWWHRA-UHFFFAOYSA-N Synonym: 2-(N-Morpholino)ethanesulfonic acid PubChem CID: 78165 ChEBI: CHEBI:39005 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid SMILES: C1COCCN1CCS(=O)(=O)O

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Invitrogen™ Glycogen (5 mg/ml)

Invitrogen™ Glycogen (5 mg/ml)

Glycogen is a branched chain carbohydrate and is useful as a nucleic acid coprecipitant.

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